Do silver ions kill bacteria Lab Report Example | Topics and Well Written Essays - 250 words

Do silver ions kill bacteria - Lab Report Example continue at 37C in a shaker incubator for over dark culture so that the bacteria would be in log phage. afterwards that spin cultures at 6000 PRM for 10 minutes. Remove supernatant, leaving 5 ml in separately tube. Resuspend pellet in the remaining supernatant and come 1ml of sterile nutrient broth to disposable plastic curvette and blank spectrophotometer. Add 1ml E.coli culture to plastic curvette and record A600 ( feature sure A600 0.5). For each Ag+ compound (WI, WII, WIII, WIV, and WV) and +Ve ascendence, groom 10 ml dilution. Prepare 17 autoclave from liquid culture tubes from nutrient broth. The final volume is 10 ml.Add 50 ml of E. coli for each different compound of Ag+ silver ions. In the same way, add 50 ml of S. aureus for each different compound of Ag+ silver ions. For each compound of silver ion one needs to incubate at 370 C in zero time, 4 time and 24 time to try to mend a Time serious at the effect concentratio n to take samples at 0 hour, 4 hour and 24 hour to try limit a Time Concentration for Lethal does. At zero time for small tubes for each compound of silver ions and control, make dilution at 11, 1102, 1103 Spread plate. 50 l of circled dilutions above on nutrient agar plates. Spread 50 l of ve control (no bug) controls. Incubate over night culture at 37 C.At 4 times for small tubes of each compound of silver ions and control, prepare before taking sample from them and make dilution at 11, 1102, 1103. Spread plate. 50 l of circled dilutions above on nutrient agar plates. Spread 50 l of ve control (no bug) controls. Incubate over night culture at 37 C.At 24 time for small tubes of each compound of silver ions and control what I prepared before take sample from them and make dilution at 102, 1103, 1104, 1105, 1106. Spread plate. 50 l of circled dilutions above on nutrient agar plates. Spread 50 l of ve control (no bug) controls. Incubate over night culture at 37